Cov tib neeg tau txaus siab los hloov fatty acid esters rau hauv dicarboxylic acids. Txawm li cas los xij, ob peb tsab ntawv ceeb toom tau hais txog kev ua sebacic acid li no. Decanoic acid yog tshuaj lom, uas yog ib qho nyuaj rau cov txheej txheem no.
Txoj kev tshawb no saib seb cov intermediates no cuam tshuam li cas rau cov txheej txheem biotransformation. Txawm tias me me ntawm decanoic acid nres qhov kev hloov pauv ntawm 10-hydroxydecanoic acid rau sebacic acid. Peb tau saib yuav ua li cas decanoic acid thiab 10-hydroxydecanoic acid cuam tshuam rau txoj kev ,ω-oxidation. Txhawm rau ua kom ntau sebacic acid, peb tau sim tshem tawm ntawm kev tsim los ntawm kev siv decane induction nrog txoj kev pub noj tsis tu ncua. Peb tswj cov decane pub tus nqi kom tsis muaj decane nyob rau hauv lub fermenter broth. Peb siv ib tug tsawg tus nqi ntawm substrate induce sebacic acid ntau lawm.
Lub hom phiaj ntawm txoj kev tshawb no yog los txiav txim siab cov nyhuv ntawm decanoic acid thiab {{{0}}}hydroxydecanoic acid, tshwj xeeb tshaj yog cov tom kawg compound, uas accumulates thaum lub sij hawm induction, nyob rau hauv ,ω-oxidation txoj kev. Qhov no yog vim tias tam sim no muaj cov ntaub ntawv tsis txaus ntseeg ntawm lub ncauj lus no. Thaum xub thawj, qhov tshuaj lom ntawm decanoic acid tau soj ntsuam hauv cov kab lis kev cai ntawm lub raj mis thaum lub sij hawm tsim cov sebacic acid los ntawm 10-hydroxydecanoic acid (Fig. 2). Thaum tsis muaj decanoic acid, C. tropicalis tau pom kom hloov tag nrho 1 g / L ntawm 10-hydroxydecanoic acid rau sebacic acid tom qab 5 teev hauv cov kab lis kev cai. Txawm li cas los xij, txawm tias nyob rau ntawm qhov siab ntawm 0.2 g / L, decanoic acid tau pom tias inhibit kev hloov pauv ntawm 10-hydroxydecanoic acid rau sebacic acid. Tsis tas li ntawd, kev hloov pauv ntawm {{10}}hydroxydecanoic acid yog ze li qhov tsis paub tseeb thaum qhov concentration ntawm decanoic acid ntau dua 0.4 g / L. Cov txiaj ntsig no tau pom tias decanoic acid tua cov hlwb thaum nws cov concentration siab dhau (0.4 g / L).
Kev tshuaj xyuas ntawm cov ntaub ntawv qhia tau hais tias ob qho tib si nruab nrab muaj kev cuam tshuam tsis zoo rau kev tsim cov tshuaj sebacic acid. Yog li, cov xwm txheej no yuav tsum tau muab tshem tawm txhawm rau txhawm rau txhim kho kev ntseeg tau ntawm cov txheej txheem.
Hauv kev xaus, decane induction los ntawm kev pub noj tas li yog ib kauj ruam tseem ceeb hauv kev hloov pauv loj ntawm methyl decanoate rau sebacic acid. Txawm li cas los xij, qhov sib xyaw ua ke ntawm 10-hydroxydecanoic acid tseem pom tau txawm tias nyob rau hauv cov xwm txheej decane-limiting. Qhov kev tshawb pom no yuav yog ib qho qhia ntxov tias oxidation ntawm 10-hydroxydecanoic acid sawv cev rau lub fwj hauv cov txheej txheem thaum siv C. tropicalis. Txhawm rau txhim kho cov khoom tsim tau ntawm sebacic acid, kev tshawb nrhiav ntxiv rau cov xwm txheej no tuaj yeem ua pov thawj tsis muaj nuj nqis.