Cov ntawv thov tseem ceeb ntawm butadiene yog cov roj hmab hluavtaws (SBR, SBS, thermoplastic roj hmab, thiab lwm yam), dav siv hauv kev tsim cov khau khau, cov log tsheb thiab lwm yam khoom siv rau kev lag luam tsheb, cov nplaum thiab cov sealants, asphalt thiab polymer hloov kho thiab cov tebchaw rau lub hom phiaj tsis kawg.
Peb tau pom yav dhau los tias butadiene monoxide (BM), thawj cov metabolite ntawm 1,3-butadiene, reacted nrog nucleosides los tsim cov khoom alkylation uas nthuav tawm cov nqi sib txawv ntawm kev tsim thiab sib txawv nyob rau hauv vitro physiological mob. Hauv kev tshawb fawb tam sim no, BM tau hnov mob nrog ib leeg -stranded (ss) thiab ob-stranded (ds) calf thymus DNA thiab cov khoom alkylation tau tshwm sim tom qab enzymatic hydrolysis ntawm DNA. Cov khoom tseem ceeb yog regioisomeric N-7-guanine adducts. N-3-(2-hydroxy-3-buten-1-yl) adenine thiab N-3-(1-hydroxy-3-buten-2-yl) adenine, uas tau depurinated los ntawm DNA sai dua N-7-guanine adducts, kuj tau tsim.
Tsis tas li ntawd, N6-(2-hydroxy-3-buten-1-yl) deoxyadenosine thiab N6-(1-hydroxy-3-buten-2-yl) deoxyadenosine tau tshawb pom thiab cov pov thawj tau txais tias cov adducts no tau tsim los ntawm Dimroth rearrangement ntawm cov sib thooj N-1-deoxyadenosine adductky, tab sis tsis yog tom qab kev tso tawm ntawm DNA, nucleosides los ntawm enzymatic hydrolysis. N-3-(2-hydroxy-3-buten-1-yl) deoxyuridine adducts, uas tau pom meej tsim tom qab deamination cov tshuaj tiv thaiv ntawm cov deoxycytidine adducts, kuj tau kuaj pom thiab ruaj khov hauv DNA. Adduct tsim yog linearly nyob ntawm BM concentration (10-1000 mM), nrog rau adduct ratios zoo sib xws ntawm ntau yam BM concentrations. Ntawm qhov siab BM concentration (750 mM), cov adducts tau tsim nyob rau hauv ib tug linear zam mus txog 8 h nyob rau hauv ob qho tib si ssDNA thiab dsDNA. Txawm li cas los xij, tus nqi ntawm kev tsim N-3-deoxyuridine thiab N6-deoxyadenosine adducts nce 10- txog 20-fold hauv ssDNA piv rau dsDNA, thaum N-7-guanine adducts nce me ntsis, txawm tias vim qhov sib txawv ntawm hydrogen bonding hauv ssDNA piv rau ds. Cov txiaj ntsig no tuaj yeem ua rau muaj kev nkag siab zoo dua ntawm cov txheej txheem molecular ntawm mutagenesis thiab carcinogenesis ntawm ob qho tib si BM thiab nws cov niam txiv compound, 1,3-butadiene.
Thaum 1,3-butadiene yog incubated nrog daim siab postmitochondrial feem ntawm nas, nas, liab los yog txiv neej thiab ib tug NADPH-regenerating system, qhov tsim ntawm butadiene monoxide yog txawv nyob rau hauv plaub hom. Nrog rau kev zam ntawm rhesus liab, tus nqi ntawm epoxide yog proportional rau cov kev ua ntawm monooxygenase. Cov kab ke ntawm epoxide tsim yog B6C3F1 nas, Sprague Dawley nas, txiv neej, rhesus liab. Qhov piv ntawm nas thiab liab yog li ntawm 7∶1. Thaum 1,3-butadiene yog incubated nrog homogenates los ntawm lub ntsws cov ntaub so ntswg, tsuas yog cov ntaub so ntswg los ntawm nas thiab nas ua rau ntsuas tau butadiene monoxide concentrations. Kev ua haujlwm ntawm monooxygenase hauv ntsws cov ntaub so ntswg ntawm tus nas tsuas yog 1/30 uas nyob rau hauv nas lub siab. Los ntawm qhov sib txawv, cov ntaub so ntswg ntsws tsim cov epoxide ntau piv rau cov uas tsim los ntawm daim siab cov ntaub so ntswg, whereas liab thiab tib neeg lub ntsws cov ntaub so ntswg tsis tsim ib tug ntsuas ntawm butadiene monoxide. Cov ntaub ntawv yuav qhia tau tias cov txiaj ntsig ntawm kev tshawb fawb nas tsuag tsis ntev los no nrog 1,3-butadiene tuaj yeem tsis tuaj yeem muab ntxiv rau tus txiv neej.