Sarcosine, los yog N-methyl-glycine, yog ib qho amino acid uas tsim los ua ib qho nruab nrab hauv cov metabolism ntawm choline hauv lub raum thiab lub siab. Nws yog tsim los ntawm methylation ntawm glycine siv S-adenosylmethionine thiab enzyme N-methyltransferase.
Prostate cancer yog qhov kev loj hlob ntawm cov hlwb uas tsis muaj kev tswj hwm hauv prostate, lub caj pas hauv cov txiv neej kev ua me nyuam hauv qab lub zais zis. Kev loj hlob txawv txav ntawm cov ntaub so ntswg prostate feem ntau yog kuaj pom los ntawm kev tshuaj ntsuam xyuas, feem ntau kuaj ntshav uas kuaj xyuas cov qib prostate tshwj xeeb antigen.
Ntau, complex molecular txheej xwm characterize cancer kev loj hlob thiab kev loj hlob1,2. Kev txiav txim siab cov tes hauj lwm molecular uas paub qhov txawv ntawm cov kab mob hauv nruab nrog cev los ntawm tus kab mob metastatic tuaj yeem ua rau kev txheeb xyuas cov biomarkers tseem ceeb rau kev mob qog noj ntshav thiab kab mob aggressiveness. Txawm hais tias cov noob caj noob ces thiab cov protein qhia tau nthuav dav hauv tib neeg cov qog, me ntsis paub txog kev hloov pauv hauv ntiaj teb metabolomic uas ua rau muaj kev loj hlob neoplastic. Siv kev sib xyaw ua ke ntawm high-throughput kua-thiab-gas-chromatography-raws li huab hwm coj spectrometry, peb profiled ntau tshaj 1,126 metabolites thoob 262 cov qauv kuaj mob ntsig txog mob qog noj ntshav prostate (42 cov ntaub so ntswg thiab 110 txhua cov zis thiab ntshav). Cov txheej txheem metabolic tsis txaus ntseeg no tuaj yeem paub qhov txawv ntawm benign prostate, kho mob qog noj ntshav hauv zos thiab kab mob metastatic.
Sarcosine, ib qho N-methyl derivative ntawm cov amino acid glycine, tau txheeb xyuas tias yog ib qho metabolite sib txawv uas tau nce siab thaum lub sij hawm mob qog noj ntshav prostate mus rau metastasis thiab tuaj yeem kuaj pom tsis yog invasively hauv cov zis. Cov qib sarcosine kuj tau nce ntxiv hauv cov kab mob qog nqaij hlav qog nqaij hlav qog nqaij hlav uas cuam tshuam rau benign prostate epithelial hlwb. Knockdown ntawm glycine-N-methyl transferase, lub enzyme uas tsim sarcosine los ntawm glycine, attenuated mob qog noj ntshav prostate. Ntxiv ntawm exogenous sarcosine los yog knockdown ntawm lub enzyme uas ua rau sarcosine degradation, sarcosine dehydrogenase, induced ib tug invasive phenotype nyob rau hauv benign prostate epithelial hlwb. Androgen receptor thiab ERG noob fusion khoom sib koom ua ke tswj cov khoom ntawm txoj kev sarcosine. Ntawm no, los ntawm kev txheeb xyuas cov kev hloov pauv hauv cov metabolism hauv kev mob qog noj ntshav prostate, peb nthuav tawm sarcosine ua ib qho tseem ceeb hauv metabolic intermediary ntawm mob qog noj ntshav thiab aggression.